Study on Antidiabetic Effect of Herbal Mixtures (Aju Mbaise) on Reproductive Hormones and Renal Function in Induced Type 2 Diabetes Mellitus in FemaleWistar Rats Using Response Surface Methodology

Abstract

This study was performed to determine the effect of ‘Aju Mbaise’ herbal mixture on the female reproductive hormones as well as the kidney of diabetic Wistar Rats. The study was carried out from June - December, 2019 in the Research Laboratory of the Department of Biochemistry, Faculty of Science, University of Port Harcourt, Nigeria. The experiment involved a total number of twenty-seven (27) rats weighing between 40g-50g. Samples were induced with diabetes type 2 using high-fat diet for about 8 weeks and streptozotocin at 35mg/kg body weight. Consequently, samples were administered the cocktail extract at three different concentrations of 500mg/kg, 250mg/kg, and 100mg/kg respectively. Renal function and reproductive hormones tests were conducted accordingly after twelve weeks of treatment using their specific test kits. Response surface methodology was applied based on central composite design (CCD). Results obtained showed progressive increase in the concentrations of estrogen and progesterone, and reduction in urea and creatinine concentrations as treatment progressed. Also optimum responses were obtained for studied electrolytes. This study established that the cocktail of ‘Aju Mbaise’ herbal blend demonstrated a significant hypoglycaemic proclivity in diabetic female rats.